The Phospho-MAPK14 (T180/Y182) Polyclonal Antibody (CABP0526) is a valuable tool for researchers studying the MAPK14 protein, also known as p38 MAPK. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of MAPK14 at threonine 180 and tyrosine 182 residues, crucial for its activation and function in cellular signaling pathways.MAPK14 is a key player in various cellular processes, including inflammation, stress response, and cell proliferation. Phosphorylation of MAPK14 at T180/Y182 is essential for its activation and subsequent downstream signaling events. The Phospho-MAPK14 antibody is validated for use in Western blot applications and is highly reactive with human samples, allowing for precise detection and quantification of phosphorylated MAPK14 in different cell types and experimental conditions.
Research on MAPK14 and its phosphorylation status is essential for understanding its role in various diseases, including cancer, inflammatory disorders, and neurodegenerative diseases. The Phospho-MAPK14 (T180/Y182) Polyclonal Antibody provides a reliable tool for investigating the activation and function of MAPK14 in these contexts, aiding in the development of targeted therapies and drug interventions.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
5600/6300/5603/1432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (CABP0526) at 1:18000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
