The Phospho-MAP3K7 (S412) Polyclonal Antibody (CABP0071) is a valuable tool for researchers studying the MAP3K7 protein, a key player in the MAP kinase signaling pathway. This antibody, produced in rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry.MAP3K7, also known as TAK1, is a serine/threonine kinase that activates various downstream signaling pathways involved in cell growth, differentiation, and apoptosis. Phosphorylation of MAP3K7 at S412 is known to be important for its activity and function in these pathways.
By targeting this specific phosphorylation site, researchers can gain insight into the regulation and function of MAP3K7 in different cellular processes.The Phospho-MAP3K7 (S412) Polyclonal Antibody is highly specific to human samples, allowing for accurate detection and analysis of MAP3K7 phosphorylation in different cell types and tissues. Its use in research on signaling pathways, cancer biology, and inflammatory responses makes it a valuable asset for laboratories focused on understanding the molecular mechanisms underlying these processes.
Product Name:
Phospho-TAK1-S412 Rabbit Polyclonal Antibody
SKU:
CABP0071
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S412 of human TAK1 (NP_003179.1).
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
6885
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-TAK1-S412 antibody (CABP0071) at 1:1000 dilution.C2C12 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
