The Phospho-MAP3K5 (T918) Polyclonal Antibody (CABP0061) is a valuable tool for researchers studying the MAP3K5 protein, a key player in the MAP kinase signaling pathway. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of MAP3K5 at threonine 918, this antibody allows for the detection and analysis of MAP3K5 activation in various cell types.MAP3K5 is a crucial component of signaling cascades involved in cell growth, differentiation, and apoptosis. It is known to play a role in various biological processes, including immune response regulation and development.
Dysregulation of MAP3K5 has been implicated in diseases such as cancer, cardiovascular disorders, and neurodegenerative diseases. As such, studying the phosphorylation status of MAP3K5 can provide important insights into its function and potential therapeutic implications in these disease contexts.The Phospho-MAP3K5 (T918) Polyclonal Antibody is a valuable tool for researchers interested in unraveling the intricacies of MAP3K5 signaling and its role in disease development. Its high specificity and reliability make it a trusted reagent for studies in cell biology, immunology, and cancer research.
Product Name:
Phospho-MAP3K5-T918 Rabbit Polyclonal Antibody
SKU:
CABP0061
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T918 of human MAP3K5 (Q59GL6).
Sequence:
TETF T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
ASK1; MEKK5; MAPKKK5; Phospho-MAP3K5-T918
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Endoplasmic reticulum.
Calculated MW:
155kDa
Observed MW:
130kDa
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Purification Method:
Affinity purification
Gene ID:
4217
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MAP3K5-T918 Rabbit pAb (CABP0061) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
