The Phospho-MAP3K5 (S1033) Polyclonal Antibody (CABP0058) is a vital tool for researchers studying the MAP3K5 protein and its phosphorylation at serine 1033. MAP3K5, also known as apoptosis signal-regulating kinase 1 (ASK1), is a key player in the MAPK signaling pathway, regulating diverse cellular processes such as apoptosis, inflammation, and stress responses.This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for Western blot applications. By targeting the phosphorylated form of MAP3K5, researchers can investigate the activation of MAPK signaling cascades and the downstream effects on cellular physiology and pathology.
MAP3K5 phosphorylation at serine 1033 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. Understanding the regulatory mechanisms of MAP3K5 activation can provide valuable insights into disease processes and potential therapeutic targets.Overall, the Phospho-MAP3K5 (S1033) Polyclonal Antibody is a valuable tool for researchers delving into the complex mechanisms of MAPK signaling and its role in health and disease. Its specificity and sensitivity make it a reliable reagent for studying MAP3K5 phosphorylation dynamics in various biological contexts.
Product Name:
Phospho-MAP3K5-S1033 Rabbit Polyclonal Antibody
SKU:
CABP0058
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S1033 of human MAP3K5 (NP_005914.1).
Sequence:
PPSP E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
ASK1; MEKK5; MAPKKK5; Phospho-MAP3K5-S1033
Positive Sample:
A-431,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Endoplasmic reticulum.
Calculated MW:
155kDa
Observed MW:
155kDa
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Purification Method:
Affinity purification
Gene ID:
4217
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 and HeLa cells, using Phospho-MAP3K5-S1033 Rabbit pAb (CABP0058) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
