The Phospho-MAP2K6 (S207) / MAP2K3 (S189) Polyclonal Antibody (CABP0081) is a valuable tool for researchers studying the MAP2K6 and MAP2K3 proteins, which play key roles in cell signaling pathways involved in various cellular processes. This antibody, produced in rabbits, is highly specific for detecting phosphorylated forms of MAP2K6 at serine 207 and MAP2K3 at serine 189 in human samples.The phosphorylation of MAP2K6 and MAP2K3 is known to regulate their activity, leading to downstream effects on cell growth, differentiation, and apoptosis. This antibody is validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated MAP2K6 and MAP2K3 in different cell types.
Research involving MAP2K6 and MAP2K3 is important in understanding how these proteins contribute to normal cellular function as well as their involvement in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. By studying the phosphorylation of MAP2K6 at serine 207 and MAP2K3 at serine 189, researchers can gain insights into the signaling pathways controlled by these proteins and potentially identify new therapeutic targets for these diseases.
A synthetic phosphorylated peptide around S207 of human MAP2K6 (NP_002749.2).
Sequence:
VDSV A
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MAP2K3/MAP2K6; Phospho-MAP2K6-S207/MAP2K3-S189
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
cytosol, nucleoplasm
Calculated MW:
36kDa/39kDa/31kDa/37kDa
Observed MW:
36kDa
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5606/5608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-MAP2K6-S207/MAP2K3-S189 Rabbit pAb (CABP0081) at 1:500 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
