The Phospho-MAP2K4 (T261) Polyclonal Antibody (CABP0069) is a valuable tool for researchers studying the MAP2K4 protein and its phosphorylation at threonine 261. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.MAP2K4 is a crucial component of the MAPK signaling pathway, which regulates various cellular processes including proliferation, differentiation, and apoptosis. Phosphorylation of MAP2K4 at threonine 261 has been associated with specific cellular responses and may play a role in cancer progression and other diseases.
By using the Phospho-MAP2K4 (T261) Polyclonal Antibody, researchers can detect and analyze the phosphorylation status of MAP2K4 in different cell types, providing valuable insights into its function and potential therapeutic implications. This antibody is an essential tool for studies in cell signaling, cancer biology, and drug development, offering new opportunities for understanding and targeting MAP2K4-mediated pathways.
Product Name:
Phospho-MAP2K4-T261 Rabbit Polyclonal Antibody
SKU:
CABP0069
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T261 of human MAP2K4 (NP_003001.1).
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6416
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-MAP2K4-T261 pAb (CABP0069) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes or treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
