The Phospho-MAP2K4 (S257/T261) Polyclonal Antibody (CABP0541) is a valuable tool for researchers studying the MAP2K4 protein and its phosphorylation at serine 257 and threonine 261 residues. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.MAP2K4, also known as Mitogen-activated protein kinase kinase 4, plays a key role in the MAPK signaling pathway, regulating cellular responses to various stimuli such as growth factors, cytokines, and stress signals. Phosphorylation of MAP2K4 at serine 257 and threonine 261 is known to modulate its activity, affecting downstream signaling pathways involved in cell growth, differentiation, and apoptosis.
Researchers in the fields of cell biology, cancer research, and signal transduction will find this antibody useful for detecting and analyzing phosphorylated MAP2K4 in different cell types and experimental conditions. Understanding the regulation of MAP2K4 phosphorylation can provide insights into its functional role in specific cellular processes and its potential as a therapeutic target for diseases associated with dysregulated MAPK signaling.
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6416
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K4-S257/T261 antibody (CABP0541) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
