The Phospho-MAP2K4 (S257) Polyclonal Antibody (CABP0068) is a valuable tool for researchers studying MAP2K4, a key player in cellular signaling pathways. This antibody, generated in rabbits, is highly specific for detecting phosphorylated MAP2K4 at serine 257 in human samples, making it ideal for Western blot analysis. By targeting this phosphorylation site, researchers can gain insights into the regulation of MAP2K4 activity and its role in various biological processes.MAP2K4, also known as Mitogen-Activated Protein Kinase Kinase 4, is involved in signaling cascades that control cell growth, differentiation, and survival. Phosphorylation at serine 257 is a crucial event that modulates MAP2K4 function, impacting downstream pathways and cellular responses.
Understanding the dynamic regulation of MAP2K4 phosphorylation can provide valuable insights into diseases such as cancer, where dysregulated signaling pathways contribute to pathogenesis.With its high specificity and sensitivity, the Phospho-MAP2K4 (S257) Polyclonal Antibody (CABP0068) is a powerful tool for researchers investigating MAP2K4 signaling in various cellular processes and disease states. Its ability to detect phosphorylated MAP2K4 at serine 257 enables precise analysis of MAP2K4 activation, offering opportunities for new discoveries in the fields of molecular biology and medicine.
Product Name:
Phospho-MAP2K4-S257 Rabbit Polyclonal Antibody
SKU:
CABP0068
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S257 of human MAP2K4 (NP_003001.1).
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6416
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K4-S257 pAb (CABP0068) at 1:1000 dilution or MAP2K4 antibody (CAB14781).HeLa cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
