The Phospho-MAP2K3 (S189)/MAP2K6 (S207) Polyclonal Antibody (CABP1112) is a valuable tool for researchers studying the phosphorylation status of MAP2K3 and MAP2K6 proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.MAP2K3 and MAP2K6 are key components of the MAPK signaling pathway, which plays a critical role in various cellular processes including cell growth, differentiation, and survival. Phosphorylation of these proteins at specific sites, such as S189 on MAP2K3 and S207 on MAP2K6, can regulate their activity and downstream signaling events.By using the Phospho-MAP2K3 (S189)/MAP2K6 (S207) Polyclonal Antibody, researchers can investigate the phosphorylation status of these proteins in different cell types and under various conditions.
This antibody enables the detection and analysis of phospho-MAP2K3 and phospho-MAP2K6, providing valuable insights into their role in signaling pathways related to cellular proliferation, differentiation, and disease progression.Overall, this antibody is a crucial tool for studies focused on understanding the molecular mechanisms underlying MAPK signaling and its implications in diseases such as cancer, neurodegenerative disorders, and immune-related conditions. Its specificity and reliability make it a valuable asset for researchers
A synthetic phosphorylated peptide around S207 of human MAP2K3/MAP2K6MAP2K3 (NP_002747.2).
Sequence:
VDSV A
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
NIH/3T3
Cellular Localization:
cytosol, nucleoplasm
Observed MW:
38kDa
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5606/ 5608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MAP2K3-S189 + MAP2K6-S207 Rabbit pAb (CABP1112) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
