The Phospho-MEK1-T286 Antibody (CABP0065) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-MEK1-T286 Antibody
SKU:
CABP0065
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with ATP, NIH/3T3 treated with Nocodazole, NIH/3T3 treated with ATP, PC-12 treated with Nocodazole, PC-12 treated with ATP
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method
Affinity purification
Gene ID
5604
RRID
AB_2771283
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (CABP0065) at 1:2000 dilution or MEK1 antibody (A12687). HeLa cells were treated with nocodazole (50 ng/mL) at 37℃ for 20 hours or treated with ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours or treated with ATP(5 mM) at 30℃ for 1 hour. PC-12 cells were treated with Nocodazole (1 μg/mL) at 37℃ for 16 hours or treated with ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (). Exposure time: 30s.
