The Phospho-MAP2K1 (S298) Polyclonal Antibody (CABP0063) is a valuable tool for researchers studying MAP2K1, a critical protein involved in cell signaling pathways. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for detecting phosphorylation of MAP2K1 at serine 298 in human samples. Validated for use in Western blot applications, this antibody enables precise detection and quantification of phosphorylated MAP2K1 in various cell types.MAP2K1, also known as MEK1, is a key kinase that regulates important cellular processes such as cell growth, differentiation, and survival. Phosphorylation of MAP2K1 at serine 298 is a crucial post-translational modification that modulates its activity and downstream signaling cascades.
Understanding the role of phosphorylated MAP2K1 in signaling pathways is essential for unraveling its involvement in diseases like cancer, where dysregulation of MAP2K1 signaling is common.Researchers studying cell signaling, cancer biology, and molecular pathways will find the Phospho-MAP2K1 (S298) Polyclonal Antibody (CABP0063) to be a reliable tool for investigating the functional significance of phosphorylated MAP2K1 in various biological contexts. Its specificity and sensitivity make it a valuable asset for unraveling the complexities of MAP2K1 signaling and its potential as a therapeutic target in cancer research.
Product Name:
Phospho-MEK1-S298 Rabbit Polyclonal Antibody
SKU:
CABP0063
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S298 of human MEK1 (NP_002746.1).
Sequence:
PLSS Y
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (CABP0063) at 1:1000 dilution or MEK1 antibody (CAB12687). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
