The Phospho-MAP2K1 (S217) Polyclonal Antibody (CABP0062) is a valuable tool for researchers studying MAP2K1, a critical regulator in cell signaling pathways. This antibody, raised in rabbits, offers high specificity and sensitivity for detecting phosphorylation of MAP2K1 at serine 217. Validated for use in Western blot applications with human samples, it allows for precise detection and quantification of MAP2K1 phosphorylation levels in various cell types.MAP2K1, also known as MEK1, is a key component in the MAPK/ERK signaling pathway, which plays a crucial role in cell proliferation, differentiation, and survival. Phosphorylation of MAP2K1 at serine 217 can impact the activation of downstream signaling cascades, ultimately influencing cellular responses.
The Phospho-MAP2K1 (S217) Polyclonal Antibody enables researchers to investigate the regulation and function of phosphorylated MAP2K1, providing insights into its roles in cancer, development, and other cellular processes.By utilizing this antibody in their studies, researchers can gain a better understanding of the intricate mechanisms underlying MAP2K1 signaling and its implications in various physiological and pathological conditions. The precise detection of MAP2K1 phosphorylation at serine 217 opens up new avenues for exploring potential therapeutic targets and developing novel strategies for treating diseases associated with dysregulated MAPK/ERK signaling.
Product Name:
Phospho-MEK1-S217 Rabbit Polyclonal Antibody
SKU:
CABP0062
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S217 of human MEK1 (NP_002746.1).
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-MEK1-S217 pAb (CABP0062) at 1:1000 dilution or MEK1 antibody (CAB12687).HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
