The Phospho-MAP2K1 (S217) & MAP2K2 (S221) Polyclonal Antibody (CABP0209) is a valuable tool for researchers studying MAP2K1 and MAP2K2, key kinases involved in the MAPK signaling pathway. This antibody, produced in rabbits, is highly specific for phosphorylated MAP2K1 at Serine 217 and MAP2K2 at Serine 221 in human samples, making it ideal for Western blot applications.Activation of MAP2K1 and MAP2K2 has been linked to various cellular processes, including cell proliferation, differentiation, and apoptosis. Phosphorylation at specific sites, such as Serine 217 and Serine 221, is crucial for their activation and downstream signaling cascades.
By targeting these phosphorylation sites, researchers can gain insights into the regulation and function of MAP2K1 and MAP2K2 in different cell types and physiological conditions.The Phospho-MAP2K1 (S217) & MAP2K2 (S221) Polyclonal Antibody is a valuable tool for studies in cell signaling, cancer research, and drug development. Understanding the role of MAP2K1 and MAP2K2 phosphorylation in cellular pathways can provide important insights into disease mechanisms and potential therapeutic targets. By detecting and analyzing phosphorylated MAP2K1 and MAP2K2, researchers can further our understanding of the intricate MAPK signaling
A synthetic phosphorylated peptide around S217 & S221 of human MAP2K1/MAP2K2MAP2K1 (NP_002746.1).
Sequence:
SMAN S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:2000 - 1:7000
Synonyms:
MEK1/MEK2; Phospho-MAP2K1-S217/MAP2K2-S221
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
cytosol, early endosome, endoplasmic reticulum, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, nucleus, plasma membrane
Calculated MW:
40kDa/43kDa/44kDa
Observed MW:
44kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5604/5605
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (CABP0209) at 1:6000 dilution. HeLa cells were treated by PMA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Enhanced Kit (AbGn00021).Exposuretime: 90s.
