The Phospho-MAP2K1 (pS221) Polyclonal Antibody (CABP0064) is a valuable tool for research involving MAP2K1, a key signaling protein in the MAP kinase pathway. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications. It specifically recognizes MAP2K1 phosphorylated at serine 221, allowing for the detection and analysis of this critical post-translational modification in various cell types.MAP2K1, also known as MEK1, plays a crucial role in cell signaling pathways that regulate cell growth, differentiation, and survival.
Phosphorylation of MAP2K1 at serine 221 is known to affect its activity and downstream signaling, making it an important target for research in cancer biology, cell signaling, and drug development. Understanding the role of phosphorylated MAP2K1 in cellular processes is essential for advancing our knowledge of disease mechanisms and potential therapeutic interventions.
Product Name:
Phospho-MEK1-pS221 Rabbit Polyclonal Antibody
SKU:
CABP0064
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S221 of human MEK1 (NP_002746.1).
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-MEK1-pS221 antibody (CABP0064) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
