The Phospho-Lyn-Y396 Monoclonal Antibody (CABP1050) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is highly specific for detecting Lyn phosphorylated at tyrosine 396 in human samples and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can accurately study Lyn activation and its downstream effects in various cell types.Lyn, a member of the Src family of kinases, plays a crucial role in regulating immune responses, cell growth, and differentiation. Phosphorylation of Lyn at tyrosine 396 is known to be important for its kinase activity and downstream signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-Lyn-Y396 Monoclonal Antibody
SKU:
CABP1050
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC1597
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
DNEY T
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4067
RRID
AB_2863923
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y397 Rabbit mAb (CABP1050) at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
