The Phospho-Lyn (Y396) Rabbit Monoclonal Antibody (CABP1050) is a valuable tool for researchers studying Lyn, a tyrosine kinase involved in cell signaling pathways. This antibody is highly specific for detecting Lyn phosphorylated at tyrosine 396 in human samples and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can accurately study Lyn activation and its downstream effects in various cell types.Lyn, a member of the Src family of kinases, plays a crucial role in regulating immune responses, cell growth, and differentiation. Phosphorylation of Lyn at tyrosine 396 is known to be important for its kinase activity and downstream signaling cascades.
Understanding the regulation of Lyn phosphorylation can provide insights into its function in immune responses, cancer progression, and inflammatory diseases.This antibody is a valuable tool for studying Lyn phosphorylation status in different experimental settings, including studies related to immunology, cancer biology, and cell signaling. By accurately detecting and quantifying phospho-Lyn (Y396), researchers can gain a better understanding of Lyn's role in health and disease, paving the way for potential therapeutic interventions targeting this kinase pathway.
Product Name:
Phospho-Lyn-Y396 Rabbit Monoclonal Antibody
SKU:
CABP1050
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y396 of human Lyn (P07948).
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4067
Clone Number:
ARC1597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y396 Rabbit mAb (CABP1050) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
