The Phospho-LMNA (S22) Polyclonal Antibody (CABP0777) is a valuable tool for researchers studying the phosphorylation of LMNA, a protein involved in nuclear structural organization and regulation of gene expression. The antibody, raised in rabbits, is highly specific for detecting phosphorylated LMNA at serine 22 in human samples and is validated for use in Western blot applications.Phosphorylation of LMNA at serine 22 has been implicated in various cellular processes, including cell cycle regulation, DNA replication, and chromatin organization. Dysregulation of LMNA phosphorylation has been linked to diseases such as progeria, muscular dystrophy, and cancer.
By utilizing this antibody, researchers can investigate the role of phosphorylated LMNA in these conditions and gain insight into potential therapeutic targets.The Phospho-LMNA (S22) Polyclonal Antibody is a valuable tool for immunology and cancer research, allowing for the precise detection and analysis of phosphorylated LMNA in various cell types. Its specificity and sensitivity make it an ideal choice for exploring the functional significance of LMNA phosphorylation and its potential as a biomarker or therapeutic target in disease settings.
Product Name:
Phospho-Lamin A/C-S22 Rabbit Polyclonal Antibody
SKU:
CABP0777
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S22 of human Lamin A (NP_005563.1).
Sequence:
PLSP T
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Purification Method:
Affinity purification
Gene ID:
4000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (CABP0777) at 1:2000 dilution or Lamin A antibody (CAB0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s.
