Anti-Phospho-LIPE-S660 pAb Antibody (CABP0853)
- SKU:
- CABP0853
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Anti-Phospho-LIPE-S660 pAb Antibody (CABP0853)
The Phospho-Lipe (S660) Polyclonal Antibody (CABP0853) is a valuable tool for researchers studying the phosphorylation of the Lipe protein. Lipe, also known as hormone-sensitive lipase, is involved in the breakdown of stored fats in the body and is a key player in lipid metabolism.This antibody, generated in rabbits, specifically recognizes the phosphorylated form of Lipe at Serine 660. It has been validated for use in Western blot applications and is highly reactive with human samples. By targeting the phosphorylated form of Lipe, researchers can gain insights into the regulation of lipid metabolism and its implications in various diseases such as obesity, diabetes, and cardiovascular disorders.
The Phospho-Lipe (S660) Polyclonal Antibody is a valuable tool for studying the function and regulation of Lipe in different cellular contexts. Its specificity for the phosphorylated form of Lipe allows for targeted analysis of this important enzyme, making it a useful asset in lipid metabolism research and drug development efforts aimed at targeting this pathway for therapeutic intervention.
| Antibody Name: | Anti-Phospho-LIPE-S660 Antibody |
| Antibody SKU: | CABP0853 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S660 of human LIPE (NP_005348.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around S660 of human LIPE (NP_005348.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QTSR S |
| Gene ID: | 3991 |
| Uniprot: | Q05469 |
| Cellular Location: | Cell membrane, Cytoplasm, Membrane, caveola, cytosol |
| Calculated MW: | 84kDa/116kDa |
| Observed MW: | 71kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody (CABP0853) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
| Synonyms: | LIPE, AOMS4, FPLD6, HSL, LHS |
| Background: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| UniProt Protein Function: | HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin. |
| UniProt Protein Details: | Protein type:EC 3.1.1.79; Lipase Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: lipid particle Molecular Function:protein binding Biological Process: lipid catabolic process; protein amino acid phosphorylation Disease: Abdominal Obesity-metabolic Syndrome 4 |
| NCBI Summary: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q05469 |
| NCBI GenInfo Identifier: | 145559491 |
| NCBI Gene ID: | 3991 |
| NCBI Accession: | Q05469.4 |
| UniProt Secondary Accession: | Q05469,Q3LRT2, Q6NSL7, |
| UniProt Related Accession: | Q05469 |
| Molecular Weight: | 84,128 Da |
| NCBI Full Name: | Hormone-sensitive lipase |
| NCBI Synonym Full Names: | lipase E, hormone sensitive type |
| NCBI Official Symbol: | LIPEÂ Â |
| NCBI Official Synonym Symbols: | HSL; LHS; AOMS4; FPLD6Â Â |
| NCBI Protein Information: | hormone-sensitive lipase |
| UniProt Protein Name: | Hormone-sensitive lipase |
| Protein Family: | Hormone-sensitive lipase |
| UniProt Gene Name: | LIPEÂ Â |
| UniProt Entry Name: | LIPS_HUMAN |
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