Anti-Phospho-LIPE-S563 pAb Antibody (CABP0851)
- SKU:
- CABP0851
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Anti-Phospho-LIPE-S563 pAb Antibody (CABP0851)
The Phospho-Lipe Antibody (S563) (PAB-CABP0851) is a powerful tool for researchers studying lipid metabolism and signaling pathways. This antibody is raised in rabbits and is highly specific for detecting phosphorylated Lipe in human samples. It has been validated for use in Western blot applications, allowing for accurate and reliable detection of phosphorylated Lipe in various cell types.Lipe, also known as hormone-sensitive lipase, plays a crucial role in the breakdown of stored triglycerides into fatty acids and glycerol. Phosphorylation of Lipe is a key regulatory mechanism that controls its enzymatic activity, making it a key player in lipid metabolism and energy homeostasis.
The Phospho-Lipe Antibody (S563) enables researchers to study the phosphorylation status of Lipe and its impact on cellular lipid metabolism in different physiological and pathological conditions.By using the Phospho-Lipe Antibody (S563), researchers can gain valuable insights into the regulation of lipid metabolism and its implications in metabolic disorders such as obesity, diabetes, and cardiovascular diseases. This antibody is a valuable tool for studies investigating the role of Lipe and its phosphorylation in lipid signaling pathways, making it essential for research in biochemistry, cell biology, and metabolic diseases.
| Antibody Name: | Anti-Phospho-LIPE-S563 Antibody |
| Antibody SKU: | CABP0851 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S853 of human HSL (NP_005348.2 , equivalent to S563 of rat HSL |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | 3T3L1, H9C2 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S853 of human HSL (NP_005348.2 , equivalent to S563 of rat HSL |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3991 |
| Uniprot: | Q05469 |
| Cellular Location: | Cell membrane, Cytoplasm, Membrane, caveola, cytosol |
| Calculated MW: | 84kDa/116kDa |
| Observed MW: | 81KDa/83KDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-HSL-S563 antibody (CABP0851) at 1:1000 dilution.3T3L1 cells were treated by lamdba-PP mixed solution (1ul) at 30'C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37'C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
| Synonyms: | LIPE, AOMS4, FPLD6, HSL, LHS |
| Background: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| UniProt Protein Function: | HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin. |
| UniProt Protein Details: | Protein type:EC 3.1.1.79; Lipase Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: lipid particle Molecular Function:protein binding Biological Process: lipid catabolic process; protein amino acid phosphorylation Disease: Abdominal Obesity-metabolic Syndrome 4 |
| NCBI Summary: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q05469 |
| NCBI GenInfo Identifier: | 145559491 |
| NCBI Gene ID: | 3991 |
| NCBI Accession: | Q05469.4 |
| UniProt Secondary Accession: | Q05469,Q3LRT2, Q6NSL7, |
| UniProt Related Accession: | Q05469 |
| Molecular Weight: | 84,128 Da |
| NCBI Full Name: | Hormone-sensitive lipase |
| NCBI Synonym Full Names: | lipase E, hormone sensitive type |
| NCBI Official Symbol: | LIPEÂ Â |
| NCBI Official Synonym Symbols: | HSL; LHS; AOMS4; FPLD6Â Â |
| NCBI Protein Information: | hormone-sensitive lipase |
| UniProt Protein Name: | Hormone-sensitive lipase |
| Protein Family: | Hormone-sensitive lipase |
| UniProt Gene Name: | LIPEÂ Â |
| UniProt Entry Name: | LIPS_HUMAN |
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