LIMK2 Rabbit Polyclonal Antibody (CAB5409)
- SKU:
- CAB5409
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | LIMK2 Rabbit Polyclonal Antibody |
SKU: | CAB5409 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LIMK2 (NP_001026971.1). |
Sequence: | MGSY LSVP AYFT SRDL FRCS ECQD SLTN WYYE KDGK LYCP KDYW GKFG EFCH GCSL LMTG PFMV AGEF KYHP ECFA CMSC KVII EDGD AYAL VQHA TLYC GKCH NEVV LAPM FERL STES VQEQ LPYS VTLI SMPA TTEG RRGF SVSV ESAC SNYA TTVQ VKEV NRMH ISPN NRNA IHPG DRIL EING TPVR TLRV EEVE DAIS QTSQ TLQL LIEH DPVS QRLD QLRL EARL APHM QNAG |
Tested Applications: | WB IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 |
Synonyms: | LIMK2 |
Positive Sample: | HepG2,HeLa,Mouse pancreas |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus, Nucleus. |
Calculated MW: | 72kDa |
Observed MW: | 80kDa |
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 3985 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |