Phospho-LIMK2-T505 Rabbit Polyclonal Antibody (CABP0388)
- SKU:
- CABP0388
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Product Name: | Phospho-LIMK2-T505 Rabbit Polyclonal Antibody |
SKU: | CABP0388 |
Size: | 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2 |
Tested Applications: | WB IF/ICC |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:100 - 1:200 |
Synonyms: | LIMK2; Phospho-LIMK2-T505 |
Positive Sample: | HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus, Nucleus. |
Calculated MW: | 72kDa |
Observed MW: | 72kDa |
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 3985 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |