Phospho-Lck-Y505 Rabbit Polyclonal Antibody (CABP0285)
- SKU:
- CABP0285
- Product Type:
- Antibody
- Applications:
- WB
- IP
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Product Name: | Phospho-Lck-Y505 Rabbit Polyclonal Antibody |
| SKU: | CABP0285 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic phosphorylated peptide around Y505 of human Lck (NP_001036236.1). |
| Sequence: | GQYQ P |
| Tested Applications: | WB IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | LSK; YT16; IMD22; p56lck; pp58lck; Phospho-Lck-Y505 |
| Positive Sample: | Jurkat |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor. |
| Calculated MW: | 58kDa |
| Observed MW: | 56kDa |
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
| Purification Method: | Affinity purification |
| Gene ID: | 3932 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Jurkat cells, using Phospho-Lck-Y505 Rabbit pAb (CABP0285) at 1:1000 dilution.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s. |
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