The Phospho-LCK (Y394) Polyclonal Antibody (CABP0182) is a critical tool for research involving the phosphorylation of the LCK protein, a member of the Src family of tyrosine kinases. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of LCK at tyrosine 394, this antibody allows for the detection and analysis of LCK activation in various cell types, making it an essential tool for studies in immunology and cancer research.
Phosphorylation of LCK at tyrosine 394 plays a key role in T-cell receptor signaling and activation, making it a crucial player in immune response regulation. Dysregulation of LCK phosphorylation has been implicated in various diseases, including cancer and autoimmune disorders, highlighting the importance of studying this process. By understanding the mechanisms behind LCK phosphorylation, researchers can uncover potential therapeutic targets for manipulating immune responses in disease contexts.
Product Name:
Phospho-LCK-Y394 Rabbit Polyclonal Antibody
SKU:
CABP0182
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y394 of human LCK (NP_005347.3).
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
3932
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LCK-Y394 Rabbit pAb (CABP0182) at 1:1000 dilution.NIH/3T3 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
