The KRT18 Polyclonal Antibody (CAB1022) is a valuable tool for researchers studying keratin 18, a type I intermediate filament protein found in epithelial cells. This antibody, produced using rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding specifically to KRT18 protein, this antibody enables the detection and analysis of KRT18 in various cell types, making it ideal for studies in cell biology, cancer research, and regenerative medicine.Keratin 18 is known for its role in maintaining the structural integrity of epithelial cells and is often used as a biomarker for epithelial cell differentiation and function. Abnormal expression of KRT18 has been linked to various diseases, including liver disease, pancreatic cancer, and inflammatory disorders.
Therefore, a thorough understanding of KRT18 expression patterns and regulation is crucial for advancing our knowledge of these diseases and developing targeted therapeutics.Researchers utilizing the KRT18 Polyclonal Antibody can benefit from its high specificity and sensitivity in detecting KRT18 protein levels, allowing for precise quantification and analysis in experimental settings. By uncovering the intricate roles of KRT18 in health and disease, this antibody facilitates groundbreaking discoveries and advancements in the field of molecular biology and biomedical research.
Product Name:
Cytokeratin 18 (KRT18) Rabbit Polyclonal Antibody
SKU:
CAB1022
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human Cytokeratin 18 (KRT18) (NP_000215.1).
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3875
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Cytokeratin 18 (KRT18) Rabbit pAb (CAB1022) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
