The Phospho-KRAS (S89) Polyclonal Antibody (CABP1168) is a valuable tool for researchers studying the phosphorylation of KRAS at serine 89, a critical event in the regulation of KRAS activity and downstream signaling pathways. This antibody, raised in rabbits, exhibits high specificity and sensitivity for human samples, offering reliable detection and analysis in Western blot applications.KRAS is a key oncogene involved in cell growth and proliferation, with mutations in KRAS being frequently observed in various cancers. Phosphorylation at serine 89 has been shown to impact KRAS function and is implicated in cancer development and progression. The Phospho-KRAS (S89) Polyclonal Antibody enables researchers to explore the role of this phosphorylation event in cancer biology, providing valuable insights for potential therapeutic interventions targeting KRAS signaling pathways.
With its ability to selectively recognize and bind to phosphorylated KRAS at serine 89, this antibody is a valuable tool for studies in cancer research, molecular biology, and cell signaling. By elucidating the mechanisms underlying KRAS phosphorylation and its implications in disease pathology, researchers can advance our understanding of cancer biology and potentially uncover novel therapeutic strategies for targeting KRAS-driven malignancies.
Product Name:
Phospho-KRAS-S89 Rabbit Polyclonal Antibody
SKU:
CABP1168
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S89 of human KRAS (NP_001356715.1).
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
Purification Method:
Affinity purification
Gene ID:
3845
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-KRAS-S89 Rabbit pAb (CABP1168) at 1:500 dilution.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.NIH/3T3 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
