The Phospho-KDR (Y1175) Polyclonal Antibody (CABP0382) is a valuable tool for researchers studying the phosphorylation of KDR, also known as Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). This antibody, raised in rabbits, is highly specific to the phosphorylated form of KDR at tyrosine 1175 and is suitable for use in Western blot applications. KDR is a key receptor involved in angiogenesis, playing a crucial role in the formation of new blood vessels. Phosphorylation of KDR at tyrosine 1175 is a critical event in the activation of downstream signaling pathways that regulate angiogenesis. The Phospho-KDR (Y1175) Polyclonal Antibody enables researchers to detect and analyze the phosphorylation status of KDR in various cell types, making it a valuable tool for studies in cancer research, cardiovascular disease, and other angiogenesis-related disorders.
Understanding the phosphorylation of KDR at tyrosine 1175 is essential for unraveling the complex signaling mechanisms involved in angiogenesis and may lead to the development of novel therapeutic strategies targeting this pathway. The Phospho-KDR (Y1175) Polyclonal Antibody is a valuable asset for researchers seeking to deepen their understanding of VEGFR2 signaling and its implications in disease.
Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
152kDa
Observed MW:
230kDa
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Purification Method:
Affinity purification
Gene ID:
3791
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (CABP0382) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
