Similarly, the KCNJ11 Polyclonal Antibody (CAB5765) provided by Assay Genie is designed for research involving KCNJ11, a potassium channel protein involved in regulating insulin secretion in pancreatic beta cells. This antibody is produced in rabbits and is highly reactive with human samples, making it ideal for Western blot applications. By binding specifically to the KCNJ11 protein, this antibody allows for accurate detection and analysis in various cell types, making it a valuable tool for studies in diabetes research and related fields.
KCNJ11 is a key player in the process of glucose-stimulated insulin secretion, making it a significant target for research into diabetes and related metabolic disorders. Understanding the function and regulation of KCNJ11 is crucial for developing new therapeutic approaches for diabetes management and treatment. The KCNJ11 Polyclonal Antibody offered by Assay Genie provides researchers with a reliable tool for studying this important protein and its role in insulin secretion and glucose homeostasis.
Product Name:
KCNJ11 Rabbit Polyclonal Antibody
SKU:
CAB5765
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 171-390 of human KCNJ11 (NP_000516.3).
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
3767
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using KCNJ11 antibody (CAB5765) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
