The Phospho-KAP1/TRIM28 (S824) Monoclonal Antibody is a valuable tool for researchers studying the phosphorylation of KAP1/TRIM28 at serine 824. This post-translational modification of the protein is involved in various cellular processes, including DNA damage response and gene silencing.Raised in mice, this antibody shows high specificity for human samples and has been validated for use in Western blot and immunofluorescence applications. It specifically recognizes the phosphorylated form of KAP1/TRIM28 at serine 824, allowing for precise detection and analysis in different cell types.The phosphorylation of KAP1/TRIM28 at serine 824 has been linked to the regulation of chromatin structure and gene expression, making it a key player in gene regulation and cell signaling pathways.
By studying this phosphorylation event, researchers can gain insights into various biological processes, including DNA repair, cell cycle control, and tumorigenesis.Overall, the Phospho-KAP1/TRIM28 (S824) Monoclonal Antibody is an essential tool for investigating the role of KAP1/TRIM28 phosphorylation in cellular physiology and disease development. Its high specificity and sensitivity make it an excellent choice for studies in cancer biology, epigenetics, and signal transduction pathways.
Product Name:
Phospho-KAP1/TRIM28-S824 Monoclonal Antibody
SKU:
CABP1393
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S824 of human KAP1/TRIM28 (NP_005753.1 ).
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Purification Method:
Affinity purification
Gene ID:
10155
Clone Number:
ARC59356
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-KAP1/TRIM28-S824 antibody (CABP1393) at 1:5000 dilution.HeLa cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
