The Phospho-JUND (S255) Rabbit Monoclonal Antibody (CABP1142) is a powerful tool for researchers studying the JUND protein and its role in cellular signaling pathways. JUND is a transcription factor that plays a crucial role in regulating gene expression in response to various stimuli, including stress, growth factors, and cytokines.This antibody specifically targets the phosphorylated form of JUND at serine 255, offering researchers a specific and sensitive tool for detecting and quantifying phosphorylated JUND in various cell types and experimental conditions. Its high reactivity with human samples, along with its validation for use in Western blot applications, make it an essential tool for studies in cell biology, signal transduction, and cancer research.
Phosphorylation of JUND at serine 255 has been linked to the regulation of gene expression and cell proliferation, making this antibody an invaluable resource for researchers interested in understanding the molecular mechanisms underlying these processes. By enabling the detection and analysis of phosphorylated JUND, this antibody provides insights that can drive forward our understanding of cellular signaling and potentially lead to the development of novel therapeutic strategies targeting JUND-related pathways.
Product Name:
Phospho-JunD-S255 Rabbit Monoclonal Antibody
SKU:
CABP1142
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S255 of human JunD (P17535).
Sequence:
GESP P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AP-1; Phospho-JunD-S255
Positive Sample:
NIH/3T3+Anisomycin,NIH/3T3+UV
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
38kDa/42kDa
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).
Purification Method:
Affinity purification
Gene ID:
3727
Clone Number:
ARC1603
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (CABP1142) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
