The Phospho-JUN D (S255) Monoclonal Antibody is a crucial tool for researchers studying the phosphorylation of JUN D at serine 255. JUN D is a transcription factor that plays a key role in regulating gene expression in response to various stimuli, including stress, cytokines, and growth factors. Phosphorylation at serine 255 is known to impact the activity and stability of JUN D, making it an important post-translational modification to study.This monoclonal antibody, produced using advanced technology, is highly specific for phosphorylated JUN D at serine 255 in human samples. It has been rigorously validated for use in Western blot applications, enabling precise detection and quantification of phosphorylated JUN D in various cell types and tissues.
Researchers in the fields of cellular signaling, cancer biology, and molecular biology will find this antibody invaluable for their studies.By providing a means to investigate the phosphorylation status of JUN D at serine 255, this antibody opens up new possibilities for understanding the regulation of gene expression and cellular responses in physiological and pathological conditions. Its reliability and specificity make it a powerful tool for uncovering the intricate mechanisms underlying cell signaling pathways and potential therapeutic targets in diseases such as cancer.
Product Name:
Phospho-JunD-S255 Monoclonal Antibody
SKU:
CABP1323
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S255 of human Phospho-JunD-S255 (NP_005345.3).
Sequence:
GESP P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
AP-1; Phospho-JunD-S255
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
38kDa/42kDa
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).
Purification Method:
Affinity purification
Gene ID:
3727
Clone Number:
ARC54895
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-JunD-S255 antibody (CABP1323) at1:2000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
