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Phospho-JunD-S255 Monoclonal Antibody (CABP1323)

SKU:
CABP1323
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-JunD-S255 Monoclonal Antibody
SKU:CABP1323
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S255 of human Phospho-JunD-S255 (NP_005345.3).
Sequence:GESP P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:AP-1; Phospho-JunD-S255
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:35kDa
Observed MW:38kDa/42kDa

The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).

Purification Method:Affinity purification
Gene ID:3727
Clone Number:ARC54895
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using Phospho-JunD-S255 antibody (CABP1323) at1:2000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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