The Phospho-JunB (S259) Polyclonal Antibody (CABP0375) is a crucial tool for researchers studying the phosphorylation of the JunB protein at serine 259. This phosphorylation event is known to play a key role in regulating the activity of JunB, a transcription factor involved in cell proliferation, differentiation, and apoptosis. The antibody, raised in rabbits, has high specificity for detecting phospho-JunB (S259) in human samples, making it suitable for use in Western blot applications.JunB is a member of the AP-1 transcription factor family and is known to be involved in the regulation of gene expression in response to a variety of stimuli, including growth factors, stress signals, and cytokines.
Phosphorylation at serine 259 has been shown to influence JunB's transcriptional activity and its interaction with other proteins, impacting various cellular processes.By using the Phospho-JunB (S259) Polyclonal Antibody, researchers can gain valuable insights into the role of JunB phosphorylation in different cellular contexts, including cancer, immune response, and development. This antibody is a valuable tool for studies aiming to understand the signaling pathways that govern JunB activity and its downstream effects on gene expression.
Product Name:
Phospho-JunB-S259 Rabbit Polyclonal Antibody
SKU:
CABP0375
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S259 of human Phospho-JunB-S259
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AP-1; Phospho-JunB-S259
Positive Sample:
HT-29,HT-29+EGF
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
45kDa
Enables sequence-specific double-stranded DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of transcription factor AP-1 complex. Biomarker of Hodgkin's lymphoma and anaplastic large cell lymphoma.
Purification Method:
Affinity purification
Gene ID:
3726
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HT29 cells, using Phospho-JunB-S259 Rabbit pAb (CABP0375).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
