The Phospho-Jun (S73) Polyclonal Antibody (CABP0047) is a valuable tool for researchers studying the phosphorylation of the Jun protein at serine 73. This antibody, raised in rabbits, specifically recognizes the phosphorylated form of Jun and is validated for use in various applications, including Western blotting.Jun is a transcription factor that plays a key role in regulating gene expression and is involved in diverse cellular processes such as proliferation, differentiation, and apoptosis. Phosphorylation of Jun at serine 73 has been linked to its activation and subsequent regulation of target gene expression.
By using the Phospho-Jun (S73) Polyclonal Antibody, researchers can investigate the role of Jun phosphorylation in different cellular contexts, providing valuable insights into the molecular mechanisms underlying various biological processes. This antibody is particularly useful for studies in signaling pathways, cancer research, and developmental biology, offering a versatile tool for probing the functional significance of Jun phosphorylation.
Product Name:
Phospho-c-Jun-S73 Rabbit Polyclonal Antibody
SKU:
CABP0047
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S73 of human c-Jun (NP_002219.1).
Sequence:
LASP E
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:
AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S73
Positive Sample:
C6
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
45-48kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-c-Jun-S73 Rabbit pAb (CABP0047) at 1:400 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
