The Phospho-Jun (S63) Polyclonal Antibody (CABP0048) is a valuable tool for researchers studying the phosphorylation of the Jun protein. Jun is a component of the AP-1 transcription factor complex, which plays a key role in cellular processes such as proliferation, differentiation, and apoptosis. This antibody, generated in rabbits, is highly specific to phosphorylated Jun at serine 63 and has been validated for use in Western blot and immunohistochemistry applications.Phosphorylation of Jun at serine 63 is known to regulate its activity and function in various cellular pathways, including those involved in oncogenesis and inflammation. By targeting this phosphorylation site, researchers can gain insights into the signaling pathways and molecular mechanisms that drive these processes.
This antibody enables the detection and analysis of phosphorylated Jun in different cell types, making it a versatile tool for studies in cancer biology, immunology, and signal transduction.The Phospho-Jun (S63) Polyclonal Antibody is a valuable resource for researchers seeking to unravel the complex role of phosphorylated Jun in cellular signaling and disease development. Its high specificity and sensitivity make it an essential component of studies aimed at understanding the molecular events that underlie cancer, inflammation, and other pathological conditions.
Product Name:
Phospho-c-Jun-S63 Rabbit Polyclonal Antibody
SKU:
CABP0048
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S63 of human c-Jun (NP_002219.1).
Sequence:
LTSP D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S63
Positive Sample:
C6+UV
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
48kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of C6, using Phospho-c-Jun-S63 Rabbit pAb (CABP0048) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
