The Phospho-Jun/Jund (S73) Antibody (PAC06094) is a valuable tool for researchers studying the phosphorylation of Jun and Jund proteins. These proteins are members of the AP-1 transcription factor complex, which plays a key role in regulating gene expression in response to various stimuli. The antibody, produced in rabbits, specifically recognizes the phosphorylated form of Jun and Jund at serine 73, allowing for the detection and analysis of their activation status in Western blot applications.Phosphorylation of Jun and Jund at serine 73 is known to be involved in the regulation of gene transcription and cell proliferation. Understanding the phosphorylation status of these proteins can provide important insights into signaling pathways that control cell growth, differentiation, and survival.
The Phospho-Jun/Jund (S73) Antibody is designed to accurately detect this specific modification, making it a valuable tool for research in cancer biology, signal transduction, and developmental biology.Overall, the Phospho-Jun/Jund (S73) Antibody offers a reliable and sensitive means to study the phosphorylation status of Jun and Jund proteins, providing valuable information for researchers interested in understanding the role of AP-1 signaling in cellular processes. Its high reactivity with human samples and validation for Western blot applications make it a versatile tool for investigating the molecular mechanisms underlying gene regulation and cell signaling pathways.
Antibody Name:
Phospho-JUN/JUND (S73/100) Antibody (PACO06094)
Antibody SKU:
PACO06094
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:10000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from human AP-1 around the phosphorylation site of S73.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of VEC A549 cells using Phospho-AP-1/Jun D (S73/100) Polyclonal Antibody.
Western Blot analysis of HuvEc cells using Phospho-AP-1/Jun D (S73/100) Polyclonal Antibody.
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
NCBI Summary:
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
