The Phospho-JNK1 (T183/Y185) + JNK2 (T183/Y185) + JNK3 (T221/Y223) Monoclonal Antibody (CABP1337) is a potent tool for studying the phosphorylation status of JNK proteins, key regulators of cellular stress responses and inflammation pathways. This antibody, developed and validated for use in Western blot applications, specifically targets the phosphorylated forms of JNK1, JNK2, and JNK3 at critical threonine and tyrosine residues.JNK proteins are members of the mitogen-activated protein kinase (MAPK) family and are involved in diverse cellular processes such as cell proliferation, differentiation, and apoptosis. Phosphorylation of specific residues in JNK proteins plays a crucial role in activating their signaling functions, leading to downstream effects on gene expression and cell fate.
This monoclonal antibody provides researchers with a reliable tool for detecting and quantifying the phosphorylated forms of JNK proteins in various cell types and experimental conditions. Its high specificity and sensitivity make it an essential reagent for studies on stress signaling, inflammation, and related pathways in both normal and disease states.Overall, the Phospho-JNK1 + JNK2 + JNK3 Monoclonal Antibody (CABP1337) is an invaluable resource for investigating the roles of JNK proteins in cellular physiology and pathology, offering new insights into potential therapeutic targets for diseases associated
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
Purification Method:
Affinity purification
Gene ID:
5599/ 5601/ 5602
Clone Number:
ARC55880
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (CABP1337) at1:2000 dilution.293T and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
