Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)
The Phospho-JNK1/2/3 (T183/T183/T221) Monoclonal Antibody (CABP0631) is a powerful tool for studying the phosphorylation status of the JNK1/2/3 proteins, which are key signaling molecules involved in various cellular processes, including cell proliferation, apoptosis, and inflammation. This antibody, generated using a unique monoclonal antibody technology, specifically detects the phosphorylation of JNK1/2/3 at threonine residues 183/183/221 in human samples.The phosphorylation of JNK1/2/3 at these specific sites is known to be crucial for the activation of the JNK signaling pathway, which in turn regulates gene expression and cell survival in response to various stimuli.
By using this antibody in Western blotting experiments, researchers can gain valuable insights into the activation status of JNK1/2/3 in different cell types and under different experimental conditions.Overall, the Phospho-JNK1/2/3 (T183/T183/T221) Monoclonal Antibody (CABP0631) is a reliable and sensitive tool for investigating the role of JNK signaling in physiological and pathological processes, making it an essential component of studies in cell biology, cancer research, and drug discovery.
Antibody Name:
Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody
Antibody SKU:
CABP0631
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3
A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
5599/5601/5602
Uniprot:
P45983/P45984/P53779
Cellular Location:
Calculated MW:
35kDa/44kDa/48kDa/27kDa/52kDa
Observed MW:
46KDa/56KDa
Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (CABP0631) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight.C6 cells were treated by Anisomycin (25 ug/mL) at 37'C for 20 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631).NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.
Synonyms:
JNK1/JNK2/JNK3
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
