The Phospho-JAK2 (Y221) Polyclonal Antibody (CABP0374) is a key tool for researchers investigating the JAK2 signaling pathway, a critical regulator of cell growth, proliferation, and differentiation. This antibody, produced in rabbits, is highly specific for the phosphorylated form of JAK2 at tyrosine 221 and is validated for use in applications such as Western blot and immunohistochemistry.JAK2 (Janus kinase 2) is a member of the JAK family of tyrosine kinases, which play essential roles in cytokine signaling and immune response pathways. Phosphorylation of JAK2 at tyrosine 221 is known to be important for its activation and downstream signaling cascade, making this antibody an invaluable tool for studying JAK2 function in various cell types and disease contexts.
Researchers focusing on hematological malignancies, myeloproliferative disorders, and immune-related diseases will find this antibody particularly useful for investigating the role of phospho-JAK2 (Y221) in disease pathogenesis and for identifying novel therapeutic targets within the JAK2 signaling pathway. With its high specificity and sensitivity, this antibody opens up new possibilities for exploring the complexities of JAK2 signaling and its implications for various disease states.
Product Name:
Phospho-JAK2-Y221 Rabbit Polyclonal Antibody
SKU:
CABP0374
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y221 of human JAK2 (NP_004963.1).
This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
3717
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from K562 cells, using Phospho-JAK2-Y221 Rabbit pAb (CABP0374) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
