The Phospho-JAK2 (Y1007) Rabbit Polyclonal Antibody (CABP0917) is a valuable tool for researchers studying the JAK2 protein, a key player in cell signaling pathways and regulation of gene expression. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot and immunohistochemistry applications.JAK2, a member of the Janus kinase family, plays a crucial role in cytokine signaling and hematopoiesis. Phosphorylation at the Y1007 site is known to activate JAK2 and is therefore an important marker for understanding its regulatory mechanisms.
The CABP0917 antibody targets this specific phosphorylation site, allowing for precise detection and analysis of JAK2 activity in various cell types.With its ability to detect phosphorylated JAK2, the CABP0917 antibody is essential for research in cancer, inflammation, and other diseases where dysregulation of JAK2 signaling is implicated. By studying the phosphorylation status of JAK2, researchers can gain valuable insights into the mechanisms underlying disease progression and identify potential therapeutic targets for intervention.
Product Name:
Phospho-JAK2-Y1007 Rabbit Polyclonal Antibody
SKU:
CABP0917
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y1007 of human JAK2 (NP_004963.1).
This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
3717
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-JAK2-Y1007 antibody (CABP0917) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
