The Phospho-JAK1 (Y1022/1023) Polyclonal Antibody (CABP0530) is a valuable tool for researchers studying the JAK1 signaling pathway, which plays a crucial role in cellular growth, proliferation, and immune response regulation. This antibody, raised in rabbits, is highly specific to phosphorylated JAK1 at tyrosine residues 1022 and 1023 and is validated for use in various applications including Western blot and immunohistochemistry.JAK1 is a key component of the Janus kinase family of proteins, which are involved in cytokine receptor signaling and activation of downstream pathways. Phosphorylation of JAK1 at tyrosine residues 1022 and 1023 is known to be important for its activity in signal transduction, particularly in the context of immune responses and inflammation.
This antibody specifically detects the phosphorylated form of JAK1, allowing for precise monitoring and analysis of JAK1 activity in different cell types and experimental conditions.Researchers studying immune regulation, cancer biology, and inflammatory diseases will benefit from using the Phospho-JAK1 (Y1022/1023) Polyclonal Antibody in their studies. By understanding the role of JAK1 phosphorylation in various cellular processes, new insights into disease mechanisms and potential therapeutic targets can be uncovered. This antibody is a valuable tool for investigating the intricate signaling pathways involved in immune response modulation and disease progression.
This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. This gene plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. This gene is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and is a therapeutic target for alleviating cytokine storms. The kinase activity of this gene is directly inhibited by the suppressor of cytokine signalling 1 (SOCS1) protein. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3716
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW264.7 cells, using Phospho-Jak1-Y1034/Y1035 Rabbit pAb (CABP0530) at 1:1000 dilution.RAW264.7 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
