The Phospho-IRS1 (S307) Polyclonal Antibody (CABP0552) is a valuable tool for researchers studying insulin receptor substrate 1 (IRS1) phosphorylation at serine 307. This phosphorylation event is known to regulate insulin signaling and metabolic pathways, making it a key target for studies related to diabetes, obesity, and metabolic disorders.Raised in rabbits, this antibody is highly specific for detecting phosphorylated IRS1 (S307) in human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of IRS1 at serine 307, researchers can better understand the role of this post-translational modification in insulin signaling and metabolic regulation.
With its high reactivity and specificity, the Phospho-IRS1 (S307) Polyclonal Antibody (CABP0552) is an essential tool for investigating the molecular mechanisms underlying insulin resistance and metabolic dysfunction. Its ability to target a specific phosphorylation site on IRS1 makes it an invaluable resource for studies aimed at developing novel therapeutic strategies for metabolic diseases.
Product Name:
Phospho-IRS1-S307 Rabbit Polyclonal Antibody
SKU:
CABP0552
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S307 of human IRS1 (NP_005535.1).
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Purification Method:
Affinity purification
Gene ID:
3667
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-IRS1-S307(Human Specific) Rabbit pAb (CABP0552) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
