The Phospho-IRS1 (S1101) Polyclonal Antibody is a valuable tool for researchers studying insulin receptor substrate 1 (IRS1) phosphorylation at serine 1101. Phosphorylation of IRS1 at this site plays a critical role in insulin signaling pathways, making this antibody essential for studies related to metabolic disorders such as diabetes.This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of IRS1 at serine 1101, researchers can investigate the downstream effects on insulin signaling and glucose metabolism in various cell types.
The Phospho-IRS1 (S1101) Polyclonal Antibody offers researchers the ability to detect and analyze the phosphorylation status of IRS1 in different experimental conditions, providing insights into the mechanisms underlying insulin resistance and metabolic dysfunction. Its applications in both basic research and drug development make it a valuable asset in the fields of endocrinology and metabolism.
Product Name:
Phospho-IRS1-S1101 Polyclonal Antibody
SKU:
CABP0954
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S1101 of human IRS1 (NP_005535.1?).
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Purification Method:
Affinity purification
Gene ID:
3667
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-IRS1-S1101 Rabbit pAb (CABP0954) at 1:1000 dilution.C2C12 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
