The Phospho-IRF3 (S396) Polyclonal Antibody (CABP0623) is a vital tool for researchers studying the phosphorylation of IRF3, a key transcription factor involved in immune response signaling pathways. This antibody, raised in rabbits, specifically recognizes the phosphorylated form of IRF3 at serine 396 in human samples, making it an essential component for investigating the activation of IRF3 in various cell types.IRF3 is a crucial regulator of the innate immune response, playing a pivotal role in the transcription of interferon genes and other immune-related genes in response to viral and bacterial infections. Phosphorylation of IRF3 at serine 396 is a key event in its activation, leading to the production of antiviral cytokines and initiating the immune response against pathogens.
The Phospho-IRF3 (S396) Polyclonal Antibody is validated for use in Western blot applications, enabling researchers to detect and analyze the phosphorylation status of IRF3 in experimental samples. Its high specificity and sensitivity make it an invaluable tool for studying the activation of IRF3 and its downstream effects in the context of viral infections, autoimmune diseases, and inflammatory conditions.By understanding the phosphorylation of IRF3 and its role in immune response regulation, researchers can gain insights into the mechanisms underlying immune activation and potentially uncover new therapeutic targets for treating infectious diseases and immune-related disorders.
Product Name:
Phospho-IRF3-S396 Rabbit Polyclonal Antibody
SKU:
CABP0623
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S396 of human IRF3 (NP_001562.1).
Sequence:
HISN S
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
IIAE7; Phospho-IRF3-S396
Positive Sample:
NIH/3T3,NIH/3T3+CA
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
45kDa
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.
Purification Method:
Affinity purification
Gene ID:
3661
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IRF3-S396 Rabbit pAb (CABP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
