The Phospho-IRF3 (S386) Rabbit Monoclonal Antibody (CABP0995) is a versatile tool for researchers studying the activation and regulation of the interferon regulatory factor 3 (IRF3) pathway. This antibody, generated in rabbits, specifically targets the phosphorylated form of IRF3 at serine 386, a key post-translational modification involved in the activation of innate immune responses.Validated for use in Western blotting and immunohistochemistry applications, the Phospho-IRF3 (S386) antibody allows for the detection and quantification of phosphorylated IRF3 in various cell types and tissues.
Its high specificity and sensitivity make it an ideal choice for studies in immunology, virology, and host-pathogen interactions.By targeting the phosphorylation status of IRF3, researchers can gain insights into the signaling pathways that drive antiviral immune responses and inflammatory processes. This antibody is essential for investigating the mechanisms underlying viral infections, autoimmune diseases, and inflammatory disorders, providing a valuable tool for dissecting the intricate molecular pathways that govern immune system function.
Product Name:
Phospho-IRF3-S386 Rabbit Monoclonal Antibody
SKU:
CABP0995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S386 of human IRF3 (Q14653).
Sequence:
ASSL E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
IIAE7; Phospho-IRF3-S386
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
55kDa
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.
Purification Method:
Affinity purification
Gene ID:
3661
Clone Number:
ARC1539
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-IRF3-S386 Rabbit mAb (CABP0995) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
