The Phospho-IRE1alpha (S724) Polyclonal Antibody (CABP0878) is a valuable tool for researchers studying the unfolded protein response (UPR) pathway and its involvement in various cellular processes. This antibody, raised in rabbits, is highly specific to the phosphorylated form of IRE1alpha at serine 724 and has been validated for use in Western blot applications.IRE1alpha is a key sensor of endoplasmic reticulum (ER) stress and plays a crucial role in activating the UPR to restore ER homeostasis. Phosphorylation of IRE1alpha at serine 724 has been linked to its activation and downstream signaling, making this antibody essential for studying the regulatory mechanisms of the UPR pathway.
By utilizing the Phospho-IRE1alpha (S724) Polyclonal Antibody, researchers can investigate the role of IRE1alpha phosphorylation in diseases such as cancer, neurodegenerative disorders, and metabolic conditions. Understanding how phosphorylation of IRE1alpha impacts cellular responses to stress can provide valuable insights into potential therapeutic strategies targeting the UPR pathway.
Product Name:
Phospho-IRE1-S724 Rabbit Polyclonal Antibody
SKU:
CABP0878
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S724 of human IRE1alpha (NP_001424.3).
Sequence:
GRHS FS
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
IRE1; IRE1P; IRE1a; hIRE1p; Phospho-IRE1-S724
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum membrane, Single-pass type I membrane protein.
Calculated MW:
110kDa
Observed MW:
126kDa
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Purification Method:
Affinity purification
Gene ID:
2081
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-IRE1-S724 antibody (CABP0878) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
