The Phospho-INSR (Y1189/Y1190) & IGF1R (Y1165/Y1166) Rabbit Polyclonal Antibody (CABP1086) is a valuable tool for researchers studying the phosphorylation of insulin receptor (INSR) and insulin-like growth factor 1 receptor (IGF1R) in various cell types. This antibody, raised in rabbits, is highly specific for detecting phosphorylation at specific tyrosine residues on these receptors, enabling precise analysis in Western blot applications.The phosphorylation of tyrosine residues on INSR and IGF1R is critical for their activation and downstream signaling pathways involved in cellular growth, metabolism, and survival. Dysregulation of these pathways has been implicated in diseases such as diabetes, cancer, and metabolic disorders.
This antibody provides researchers with a reliable tool to study the phosphorylation status of these receptors, aiding in the understanding of their role in disease progression and potential therapeutic interventions.The Phospho-INSR (Y1189/Y1190) & IGF1R (Y1165/Y1166) Rabbit Polyclonal Antibody (CABP1086) is a valuable addition to any laboratory conducting research in the fields of molecular biology, metabolic disorders, and cancer biology. Its high specificity and sensitivity make it a trusted reagent for studying the phosphorylation events of INSR and IGF1R in various experimental settings.
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
Purification Method:
Affinity purification
Gene ID:
3480/3643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (CABP1086) at 1:1000 dilution or IGF1R antibody (CAB0243).293T cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
