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Phospho-SHIP1-Y1020 Rabbit Polyclonal Antibody (CABP0778)

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SKU:
CABP0778
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€159 - €419

Description

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Phospho-SHIP1-Y1020 Rabbit Polyclonal Antibody (CABP0778)

The Phospho-INPP5D (Y1020) Polyclonal Antibody (CABP0778) is a valuable tool for researchers studying the phosphorylation of INPP5D at tyrosine 1020, a key event in signaling pathways involved in immune response regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.INPP5D, also known as SHIP-1, is a phosphatase enzyme that plays a crucial role in regulating immune cell activation and function. Phosphorylation at tyrosine 1020 is known to impact the activity of INPP5D, thereby influencing downstream signaling events in immune cells.

Understanding the phosphorylation status of INPP5D is essential for unraveling its role in immune signaling pathways and its potential as a therapeutic target in diseases such as cancer and autoimmune disorders.With its specificity for phosphorylated INPP5D at tyrosine 1020, this antibody enables researchers to investigate the regulation of this critical signaling molecule in various cell types and contexts. Its application in immunology and cancer research makes it a valuable resource for studies aiming to decipher the complex mechanisms of immune regulation and develop targeted therapies for immune-related disorders.

Product Name:Phospho-SHIP1-Y1020 Rabbit Polyclonal Antibody
SKU:CABP0778
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y1020 of human SHIP1 (NP_001017915.1).
Sequence:ENPL Y
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship; Phospho-SHIP1-Y1020
Positive Sample:Raji
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Membrane, Peripheral membrane protein.
Calculated MW:133kDa
Observed MW:145kDa

This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Deficiencies in the encoded protein, SHIP1, have been associated with Inflammatory Bowel Disease types such as Crohn's Disease and Ulcerative Colitis. Alternative splicing of this gene results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:3635
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Raji cells, using Phospho-SHIP1-Y1020 Rabbit pAb (CABP0778) at 1:1000 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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