The Phospho-IKKGamma (S376) Rabbit Polyclonal Antibody (CABP1111) is designed for research involving the phosphorylation of IKKγ, a critical regulator of NF-κB signaling pathway. This antibody, raised in rabbits, is highly specific to the phosphorylated form of IKKγ at serine 376 and is validated for use in Western blot and immunohistochemistry applications. It binds to the phosphorylated IKKγ protein, enabling detection and analysis in various cell types, making it ideal for studies in immunology, inflammation, and cancer research.
The phosphorylation of IKKγ at serine 376 plays a crucial role in the activation of NF-κB signaling pathway, a key regulator of immune and inflammatory responses. Dysregulation of this pathway has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions. Understanding the role of phosphorylated IKKγ in NF-κB activation is essential for developing targeted therapies that modulate immune and inflammatory responses in these disease contexts.
Product Name:
Phospho-IKKγ-S376 Rabbit Polyclonal Antibody
SKU:
CABP1111
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S376 of human IKKγ (NP_003630.1).
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Purification Method:
Affinity purification
Gene ID:
8517
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-IKKγ-S376 Rabbit pAb (CABP1111) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
