The Phospho-IGF1R (Y1165/1166)/INSR (Y1189/1190) Polyclonal Antibody (CABP0902) is a valuable tool for researchers studying insulin-like growth factor 1 receptor (IGF1R) and insulin receptor (INSR) phosphorylation in various cellular processes. This antibody, raised in rabbits, is highly specific to the phosphorylated forms of the IGF1R and INSR proteins and has been validated for use in Western blot applications.IGF1R and INSR are receptor tyrosine kinases that play crucial roles in the regulation of cell growth, metabolism, and survival. Phosphorylation of specific tyrosine residues in these receptors is known to modulate their signaling pathways, influencing various cellular functions.
The Phospho-IGF1R (Y1165/1166)/INSR (Y1189/1190) Polyclonal Antibody enables precise detection and analysis of phosphorylated IGF1R and INSR in a wide range of cell types, making it an essential tool for studies in signal transduction, metabolism, and cancer research.The importance of IGF1R and INSR signaling in various physiological and pathological processes, including cancer development and progression, makes this antibody a valuable asset for researchers investigating these pathways. By understanding the phosphorylation status of IGF1R and INSR, researchers can gain insights into the mechanisms underlying these processes
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
Purification Method:
Affinity purification
Gene ID:
3480/3643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 pAb (CABP0902) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
