null

Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 Rabbit Polyclonal Antibody (CABP0902)

SKU:
CABP0902
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 Rabbit Polyclonal Antibody
SKU:CABP0902
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RIGF1R (NP_000866.1).
Sequence:TDYY RK
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:IGF1R/INSR; Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:cytoplasm, nucleus, plasma membrane, T-tubule
Calculated MW:154kDa/155kDa/156kDa
Observed MW:125kDa

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Purification Method:Affinity purification
Gene ID:3480/3643
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 pAb (CABP0902) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Related Products