Phospho-IGF-IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) Polyclonal Antibody (CABP1352)
- SKU:
- CABP1352
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | Phospho-IGF-IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) Polyclonal Antibody |
| SKU: | CABP1352 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic phosphorylated peptide around IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) of human IGF. |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:1000 - 1:5000 |
| Synonyms: | HHF5; CD220; IGFR; CD221; IGFIR; JTK13; Phospho-IGF-IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) |
| Positive Sample: | HeLa |
| Conjugate: | Unconjugated |
| Cellular Localization: | axon, caveola, dendrite membrane, external side of plasma membrane, extracellular exosome, late endosome, lysosome, plasma membrane |
| Calculated MW: | 156kDa |
| Observed MW: | 102kDa |
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 3643/3480 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of HeLa, using Phospho-IGF-IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) antibody (CABP1352) at 1:2000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s. |
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