The Phospho-HSPB1 (S82) Polyclonal Antibody (CABP0041) is a valuable tool for researchers studying the phosphorylation of Heat Shock Protein Beta-1 (HSPB1) at serine 82. This antibody, generated in rabbits, is highly specific to the phosphorylated form of HSPB1 and is suitable for use in Western blot applications with human samples.HSPB1 is a chaperone protein that plays a key role in cellular stress response and is involved in various cellular processes, including cell survival, apoptosis, and cytoskeletal organization. Phosphorylation of HSPB1 at serine 82 has been linked to its function in regulating actin dynamics and cytoskeletal rearrangements in response to stress stimuli.
By using the Phospho-HSPB1 (S82) Polyclonal Antibody, researchers can analyze the phosphorylation status of HSPB1 in different cell types and conditions, advancing our understanding of the molecular mechanisms underlying stress responses and cellular signaling pathways. This antibody is a valuable tool for studies in cell biology, neurodegenerative diseases, and cancer research, where dysregulation of HSPB1 phosphorylation may play a role in disease progression.
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Purification Method:
Affinity purification
Gene ID:
3315
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit pAb (CABP0041) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
